Assessment of Spermatic Chromatin Decondensation by the Toluidine Blue Assay in Infertile Patients in Cotonou

Background: A high level of sperm chromatin decondensation results in loss DNA quality and it is associated with poor embryonic prognosis Assisted Reproductive Technology (ART). This had lead to introduction compaction testing assessment clinical male infertility. Toluidine blue assay one most commonly used tests measure research studies also routine use. So the objective our study was assess by toluidine men dealing couple Methods: The samples 96 patients referred for infertility, over a 4-month period from September 2020 January 2021, were stained observed under an optical microscope. These evaluated according World Health Organization. Results: 25 - 34 age group represented among infertile men. There non-significant correlation between spermatic condensation (r = 0.0348). Spermatic parameters averages above standards norms, apart that low vitality. decondenization affected 96% patients. Asthenozoospermia common abnormality. Among standard spermogram, only concentration spermatozoa semen weakly correlated 0.18) decondensation. Conclusion: is, on hand, parameter independent subject; other allows evaluate like classic spermogram spermocytogram. However, view probability changes nuclear intrinsic analysis process concentration, improvement test using swim-up technique image processing software possible.